Fig. 8. VASP and Mena-GFP recruitment at bacteria-induced phagocytic cups. (A) Cells were stained with anti-InlA antibody to detect bacteria (blue), Phalloidin-488 to detect F-actin (green) and anti-VASP antibody (red). (B) Overexpression of Mena increases the F-actin content of phagocytic cups. Vero cells transiently transfected with Mena-GFP were stained with anti-Listeria antibody (blue) and phalloidin-546 to detect F-actin (green). Mena-GFP is shown in red. Phagocytic cups at the site of entry present a thicker actin network (arrows), magnified in the boxed region. Merged images are shown in bottom right-hand panels. Bar, 1 µm (A) and 2 µm (B).