Fig. 1. The C-terminal domain of ninein associates with both centrioles. (A) L929 cells were transiently transfected with GFP-tagged ninein N-terminal domain, coiled-coil region and C-terminal domain (green). 2 hours after transfection, cells were fixed and centrioles labelled with either GT335 antibody or -tubulin antibody (red), demonstrating that only the C-terminal domain was targeted to centrioles. Bars, 10 µm (4 x magnification in insets). (B) L929 cells expressing GFP/Cter-ninein for 2 hours were fixed and labelled with an antibody to C-Nap1 (a), which decorates the proximal ends of both centrioles (cartoon) or to ninein (b), which decorates essentially the distal end of the mother centriole (cartoon). The GFP signals did not colocalize with the C-Nap1 labelling but colocalized with ninein to the mother centriole, suggesting that GFP/Cter-ninein might be associated with the distal end of both centrioles (same magnification as insets in A).