JCS -- Pfister et al. 118 (8): 1587 Figure IG1

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. Production of HSP60 in the mitochondria of HUVECs fixed with ice-cold acetone/methanol. The nuclei appear red after staining with the fluorescent DNA dye TOPRO-3 and excitation at 543 nm. HSP60 appears green after labelling with a FITC-conjugated monoclonal anti-HSP60 antibody and excitation at 488 nm. (a) Unstressed control cells. (b) Cells exposed to heat stress (42°C, 30 minutes). (c) Detailed view of mitochondrial localization of HSP60 in heat stressed cells.