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Fig. 4. Flow cytometric analysis of unstressed (left panels) and heat-stressed (right panels) living HUVECs. (a) Dot plots of forward scatter (cell size) against side scatter (surface granularity) showing similar size and granularity in control and heat-stressed cells. (b) Dot plots of forward scatter (cell size) against FITC fluorescence intensity (FITC FLI) showing a significant increase of cells with HSP60 surface expression from 1.1% in the control cells to 9.5% in heat-stressed cells. Cells have been incubated with the FITC labelled monoclonal antibody II-13 specific for human HSP60. (c) Histogram plots of cell counts against propidium iodide intensity showing full cell membrane integrity in control cells and heat-stressed cells. The full membrane integrity is indicated by the equal high percentage (100%) of propidium iodide negative cells in both unstressed and stressed HUVECs.