JCS -- Ma et al. 118 (8): 1607 Figure IG4

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Fig. 4. Representative localization of macroH2A on mitotic chromosomes. Immunodetection of macroH2A distribution on individual metaphase chromosomes from (A) J1 (wild type) ES cells and (B) C/C (Dnmt1^C/C) ES cells. Chromosomes were stained with DAPI (blue) to detect DNA, and with FITC (green) to detect ${alpha}$ -macroH2A antibody. Images in A and B had identical exposure times. Note enrichment of macroH2A immunostaining at telocentric centromeres in Dnmt1^C/C cells. (C) Individual mitotic cells from J1 and C/C cultures were also subjected to combined immunofluorescence to detect macroH2A and kinetochore proteins (CREST).