Fig. 4. Arrest with high Clb/Cdc28p activity maintains neck-localized septins. (A) Strains DLY7326 (GAL1p-CLB1¹⁵²) and DLY7327 (cdc42^V36T,K94E GAL1p-CLB1¹⁵²) were grown to exponential phase in sucrose-containing medium at 29°C, induced to overexpress Clb1p¹⁵² by addition of galactose (2% final concentration), and processed to visualize septin distribution after a further 4 hours. (B) Strains DLY5 (WT) and DLY5080 (cdc42^V36T,K94E) were grown to exponential phase in YEPD at 29°C and nocodazole was added to 15 µg/ml to arrest cells in mitosis. After 3 hours of arrest cells were fixed and processed to visualize septin distribution. Bar, 10 µm.