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Fig. 6. Septin organization in cln1{Delta} cln2{Delta} cells. Top panels: strains DLY5103 (WT) and DLY5102 (cln1 cln2) were grown to exponential phase in YEPD at 30°C, and the cells were fixed and processed to visualize septins. Bottom panels: strains DLY5106 (GAL1p-SWE1) and DLY5104 (cln1 cln2 GAL1p-SWE1) were grown to exponential phase in YEPsucrose at 30°C, induced to express Swe1p by addition of galactose (2% for 5 hours), and the cells were fixed and processed to visualize septins. Bar, 10 µm.