Fig. 1. Distribution of Syt IX in RBL cells stably transfected with T7-Syt IX or Flag-Syt IX-GFP. (A) RBL cells, stably transfected with T7-Syt IX or Flag-Syt IX-GFP cDNA, were grown on glass coverslips and labeled with polyclonal anti-Syt IX C2A antibodies followed by Cy3-conjugated donkey anti-rabbit IgG (a). Bars, 3 µm. (B) Cell extracts (60 µg) derived from T7-Syt IX- (1) or Flag-Syt IX-GFP (2)-expressing cells were resolved by SDS-PAGE and subjected to immunoblotting with anti-Syt IX C2A antibodies.