Fig. 2 . Time-dependent in vitro reconstitution of Golgi to chloroplast transport. An isolated ³⁵S-labeled Golgi membrane fraction (M) was incubated in the presence (+) or absence (–) of Euglena chloroplasts (Chlor) in the light for 30 or 60 minutes. Fluorographs of [³⁵S]pLHCPII immunoprecipitated from Percoll-purified chloroplasts show pLHCPII present in the Golgi membrane fraction was transported to and imported into isolated chloroplasts where it was processed to LHCPII and a 50 kDa partially processed pLHCPII polyprotein in a time and chloroplast-dependent manner.