Fig. 4. Cell size and cell cycle analyses. (A-F) Representative flow cytometry analyses of keratinocytes isolated from control (Ct) or KO epidermis of skin as indicated. (A) Light scatter dot plot of whole populations. R1, region gating smaller, blunt, basal cells; R2, region gating larger and more complex, suprabasal (suprab) cells. (B) Keratin 1 (K1; FL-1) expression of cells in R2 or R1; numbers in brackets are percent of K1-negative cells (left histograms) or K1-positive cells (right histograms) within the total population; K1-positive cells are in green and K1-negative cells in red in the light scatter dot plots. (C) Overlays of cell size (forward scatter, FSC) of the populations indicated. (D) DNA content (FL2) profiles of basal (R1) or suprabasal (R2) keratinocytes. (E) DNA content profiles of proliferative (K1–) or differentiating (K1+) cells. M2 for the G1 phase; M1 for polyploid cells. (F) Cell size (FSC) versus DNA content (FL2); a line has been drawn to help comparing FSC values. 10,000 keratinocytes were counted for every sample (see Materials and Methods). Numbers are percent of cells in each gate within each plot.