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Fig. 1. A role for Amo1 in microtubule organisation and polarity. (A) Overexpression of amo1 leads to bundling of microtubules on one side of the cell. (B) Differential interference contrast microscopy images of wild-type cells (wt) and amo1{Delta} cells from plates. (C) Tubulin immunofluorescence of wild-type and amo1{Delta} cells. (D) MBC sensitivity spot assay. Cells from indicated strains were grown to mid-exponential phase, and tenfold serial dilutions spotted on plates containing the indicated amount of MBC. (E) Calcofluor White staining of septated wild-type and amo1{Delta} cells. (F) Growth patterns of pairs of cells after cell division were monitored. The two patterns observed are shown, with percentages of total cells scored. Bar, 10 µm.