Fig. 2. Localisation of Amo1 to the nuclear surface. (A) Projection of deconvolved focal sections of live interphase and mitotic cells containing Amo1-GFP. Arrowheads indicate threads of nuclear membrane between separating nuclei at anaphase. (B) Single focal section showing that Amo1-GFP (green) localises to the nuclear periphery surrounding chromosomal DNA (blue) and nucleolus. (C) Four consecutive deconvolved focal sections showing Amo1-myc (green) and Sad1 (red) detected by immunofluorescence. (D) Amo1-GFP localisation in control (DMSO) and MBC-treated cells. E. Deconvolved projection showing live MBC-treated cells expressing GFP-tubulin (green) and Amo1-mRFP1 (red). Bar, 10 µm.