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Fig. 5. Amo1{Delta} cells are not defective in NPC organisation. (A) Alignment of S. pombe Amo1 and S. cerevisiae Nup42 sequences, showing conservation of some FG repeats (in red). Protein alignment was carried out with CLUSTALW. (B) Immunofluorescence of cells expressing Amo1-myc with anti-myc (green) and anti-Nup189 (red) antibodies. (C) NLS-LacI-GFP localisation in live wild-type and amo1{Delta} cells. (D) Nup189 immunofluorescence in wild-type and amo1{Delta} cells. Bar, 10 µm.