Fig. 2. Basic residues 149-152 are required for nuclear localization. Transfections were in L cells. (A) Confocal microscopy. (B) Immunoblotting. Lane 1: Mock; lane 2: PK; lane 3: PK-LEDGF/146-156; lane 4: PK-LEDGF/RR146-147AA; lane 5: PK-LEDGF/RKRK149-152AAAA.