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Fig. 2. Chromosomes in the meiosis I spindle. The chromosomes in live oocytes were confirmed by comparing fixed and stained oocytes. (A) Mature spindle in a live ncdgfp* oocyte. The dark region in the center of the spindle excludes NcdGFP* and was inferred to correspond to the condensed meiotic chromosomes. (B) Wild-type oocyte (Oregon R) fixed and stained with rhodamine-labeled anti-{alpha}-tubulin antibody (red) and DAPI (green). The small dots at the distal ends of the chromosome mass are the small 4th chromosomes. Bar, 5 µm.