Fig. 6. Ncd motor-associated asters at the germinal vesicle. (A) Two large fluorescent asters just outside the germinal vesicle of an ncdNKgfp* oocyte. The karyosome or endobody (arrow), around which microtubule nucleation occurred, is the dark body to the lower right, closest to the fluorescence foci. The other two dark regions are due to uneven dispersion of germinal vesicle contents into the ooplasm. (B) A cluster of large asters near the germinal vesicle of an ncdNKgfp* oocyte. (C) Two large asters in the germinal vesicle of an APL10/+ oocyte; two more large asters are in the ooplasm. The fluorescent asters migrate towards the karyosome of condensed chromosomes and nucleate microtubules at germinal vesicle breakdown. The asters are larger in ncdNKgfp* and APL10/+ or APL10 oocytes, than wild-type ncdgfp* oocytes (see Fig. 1C,D for comparison). Bar, 5 µm.