Fig. 5. Localization of Sla2-GFP is independent of the actin and microtubule cytoskeletons. (A) cdc10-129 cells expressing Sla2-GFP were grown at 25°C and then transferred to 36°C for 3 hours (left). After addition of DMSO or 40 µM latA or 25 µg/ml MBC, or 25 µg/ml MBC plus 40 µM latA (indicated by an arrow in the scheme) respectively, cells were released to permissive temperature (25°C) for an hour in the presence of the drug and then analysed for Sla2-GFP localization (right panels). (B) Quantification of cells with bipolar actin distribution at 36°C (black bar) and 30 minutes after shift down to 25°C (white and grey bars) in the presence of the different drugs. Bar, 5 µm.