Fig. 2. Caspase-dependent apoptosis in wings expressing nef. (A-F) Nef-induced caspase-dependent apoptosis in wings. Wing imaginal discs were stained with acridine orange (A-C) or antibody against active Drice (D-F). (G-L) Nef did not affect cell proliferation in wings. Wing imaginal discs were examined by BrdU incorporation assay (G-I) or by immunostaining with antibody against phosphospecific histone H3 (J-L).