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Fig. 5. RhoA is hyper-activated in Bcl6–/– macrophages. (A) A GST-Rhotekin-RBD pull-down assay was performed on WT and Bcl6–/– BMM stimulated with CSF1 for 0 and 20 minutes. Eluted proteins were examined by western blotting for associated active RhoA. Bottom panel demonstrates equivalent presentation of input protein as determined by RhoA western blotting of 30 µg RIPA lysates from the above samples. (B) Bar graph summarizes the results from three independent GST-Rhotekin-RBD pull-down assays (mean±s.d.; *P<0.05 significantly different from intensity in Bcl6+/+ BMM at 0 or 20 minutes; **P<0.05, significantly different from intensity in Bcl6+/+ BMM at 0 minute).