JCS -- Lin et al. 118 (9): 1885 Figure IG3

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Fig. 3. Co-immunoprecipitation of SCAMP2_myc deletion mutants and NHE7_HA. CHO cells were transiently co-transfected with myc-tagged SCAMP2 deletion mutants as illustrated above the blots, and HA-tagged NHE7. Pre-cleared lysates (Lys) were immunoprecipitated with an anti-HA antibody (HA) or mouse pre-immune serum (con) and bound SCAMP2 was detected on western blot by an anti-myc antibody. 5% of the lysate was run for each sample.