Fig. 4. Dose-dependent binding of SCAMP2[201-215] to NHE7. (A) Predicted membrane topology of SCAMP2. (B) Increasing amounts of GST-SCAMP2[201-215] immobilized to glutathione beads were incubated with radiolabelled NHE7 C-terminus and the bound NHE7 was visualized using a PhosphorImager. 4 µg GST was used as a control.