Fig. 2. Vglut1 and ZnT3 colocalize in PC12 cells. PC12 cells expressing ZnT3 and Vglut1 were double labeled with antibodies against the HA tag present in ZnT3 and antibodies against Vglut1. Cell were imaged by wide-field deconvolution microscopy and colocalization was determined by Metamorph software using ten randomly picked cells. (A-C) Optical sections from the bottom of the cell to the top, spaced every 0.75 µm.