Fig. 5. Zinquin colocalizes with the synaptic-vesicle marker VAMP II in differentiated PC12 cells. (A) NGF-differentiated PC12 cells transfected with the early endosome marker syntaxin-13/GFP were zinc loaded, stained with zinquin and imaged by two-photon microscopy. Zinquin puncta and syntaxin-13/GFP failed to colocalize. (B) NGF-differentiated PC12 cells accumulate zinquin in vesicular compartments that (C) co-localize with the synaptic-vesicle marker VAMP-II/GFP.