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Fig. 2. Kir2.4 accumulates in the Golgi complex. (A) Representative confocal images showing the subcellular distribution of GFPKir2.4 in OK cells. Golgi accumulation is shown by co-localization of GFPKir2.4 with DsRed2 fused to the targeting sequence of 1,4-galactosyltransferase (Golgi; upper panel) and redistribution of Kir2.4 into the ER by incubation with brefeldin A (BFA; lower panel). (B) Golgi accumulation of GFPKir2.4 is not due to retrograde transport to the Golgi complex. Extracellular epitope tagging does not reveal significant internalization of GFPKir2.4 after 16 hours (upper panel); inhibition of clathrin-dependent endocytosis by co-expression of a HA-tagged dominant-negative dynamin (dn-Dyn) mutant (dynamin-K44A) (Damke et al., 1994) does not affect the subcellular distribution of GFPKir2.4 (lower panel).