Fig. 3. Tailless subunits can form complexes with other subunits and conduct GluR function. (A) HA::GLR-1 and FLAG::GLR-2 were contransfected into COS7 cells, which were solubilized in RIPA. Immunoprecipitations (IP) were performed with the indicated antisera, and co-immunoprecipitated proteins were detected by immunoblotting (IB) with the indicated antisera. Arrows indicate the protein pulled-down. (B) HA::GLR-1 and FLAG::GLR-2(tailless), indicated as `FLAG::GLR-2T', were contransfected and analyzed as above. (C) FLAG::GLR-2 and HA::GLR-1(tailless), indicated as `HA::GLR-1T', were contransfected and analyzed as above. For (A-C), `load' indicates 10% of the original lysate. (D) The mean nose-touch sensitivity (percentage of ten trials per animal in which the animal reversed direction upon forward collision with an eyelash) is plotted for the given genotype. `GLR-1 in glr-1' indicates the presence of the glr-1::gfp transgene in a glr-1 mutant, whereas `GLR-1T in glr-1' indicates the presence of the glr-1(tailless)::gfp transgene in a glr-1 mutant. (E) The mean nose-touch sensitivity is plotted for the given genotype. `GLR-2 in glr-2' indicates the presence of the glr-2::gfp transgene in a glr-2 mutant, whereas `GLR-2T in glr-2' indicates the presence of the glr-2(tailless)::gfp transgene in a glr-2 mutant. For (D) and (E), n=13-40 animals for each genotype. ***P<0.001 by ANOVA with the indicated Bonferoni multiple comparison.