Fig. 5. GLR-2 cytosolic tail fusion protein requires the PDZ-binding motif and LIN-10 protein to be localized. We substituted the last three amino acids of the GLR-2 PDZ-binding motif (TLFALE) in TMGFP::GLR-2(tail) to generate TMGFP::GLR-2(TLFALE). (A,B) In wild-type animals, we expressed TMGFP::GLR-2(tail) (A) and TMGFP::GLR-2(TLFALE) (B). (C,D) In lin-10 animals, we expressed TMGFP::GLR-2(tail) (C) and TMGFP::GLR-2(TLFALE) (D). Both B and C show a reduced size of synaptic clusters compared with A, suggesting that both LIN-10 and the PDZ-binding motif of GLR-2 are important for synaptic localization of a GLR-2 tail chimeric protein. (D) These two pathways may compensate for each other as removal of both pathways by expressing a chimeric GLR-2 protein with a mutated PDZ-binding motif in a lin-10 background abolishes the synaptic localization pattern. Bar, 5 µm. (E) The mean number of clusters per 10 µm of ventral cord length, and (F) the mean cluster size is plotted for the given genotype. Error bars are s.e.m. for both graphs. **P<0.01 compared with TLF in wild-type by ANOVA/Dunnett's multiple comparisons test. n=15-20 animals for each genotype.