Fig. 6. Sequence motifs conserved among known GluR tail sequences. (A) The predicted amino acid sequences of five nematode GLR-1 tail sequences (C. elegans (C.e.), C. briggsae (C.b.), C. remanei (C.r.), A. suum (A.s.) and S. stercoralis (S.s.)), aligned by CLUSTALW. This sequence corresponds to amino acids 876-962 of C.e. GLR-1. Black boxes indicate identities present in the majority of sequences. Gray boxes indicate similarities present in the majority of sequences. Lines above the sequence demarcate the conserved sequence motifs identified in D and E. The S.s. GLR-1 sequence is derived from a partial EST, and therefore lacks the final 3' residues. (B) The predicted amino acids of three nematode GLR-2 tail sequences, aligned by CLUSTALW. These sequences correspond to amino acids 919-977 of C.e. GLR-2, and are labeled as in A. (C) The predicted amino acid sequence of three rat long tail AMPAR subunits (R1, R2L, R4), three rat short tail AMPARs (R4S, R3, R2), and GLR-1 and GLR-2, aligned by CLUSTALW and JalView v1.8 Multiple Alignment Editor. Gray boxes indicate identities and similarities present in three or more sequences. (D) The predicted sequence of rat and C.e. GluRs, emphasizing the indicated sequence motif. For the PKA motif, the underlined residue in GluR1 indicates the phosphorylated serine. For the regulated endocytosis motif, the underlined residues in GluR2 indicate the phosphorylated serine and tyrosines. (E) The predicted PDZ ligands for various rat GluRs aligned with GLR-1 and GLR-2. The type of PDZ ligand (I versus II) and its corresponding consensus is shown. `' indicates any hydrophobic residue.