Fig. 9. Cellular location of p38 following treatment of M2R cells with Pd-Bchl-Ser and light. (A) Cells were treated with Pd-Bchl-Ser at LD₉₀ and light. Samples were fixed and permeabilized at varying time intervals after illumination (0-45 minutes) and stained with anti-phosphorylated p38. UN, untreated cells; UN-p38, untreated cells stained with anti-p38; anti-p-p38+dP peptide, immunostaining of cells treated with anti-phosphorylated p38 pre-reacted with its immunogen, the doubly phosphorylated (dP) p38 peptide, was completely blocked demonstrating specificity of the staining. (B) As in A but cells were treated with Pd-Bchl-Ser at LD₂₀ and light.