Fig. 2. Colon lamina propria lymphocytes stained with surface markers and analyzed by flow cytometry. LPL were isolated from K8+/+ and K8–/– colons, stained with the indicated markers then analyzed as described in Materials and Methods. Markedly higher numbers of TCRß and CD4+ T cells are recovered from the K8–/– compared to K8+/+ mouse colons in an age-dependent manner. The data is presented as mean cell number±s.e.m. (n=4). *P<0.05 when comparing K8–/– with K8+/+.