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Fig. 3. PH-G domain of MTMR3 binds PtdIns5P. HeLa cells were transfected with HA-MTMR3 (A and B), HA-MTMR3-{Delta}PH-G (C) or RFP-2 xPH-G (D,E). In addition, some cells were cotransfected with EGFP-IpgD phosphatase (B-D), EGFP-IpgD staining is shown for each in the corresponding lower panel (F-H). IpgD expression results in MTMR3 translocation to the plasma membrane (B). Deletion of the PH-G domain ({Delta}PH-G) from MTMR3 abolishes translocation (C), whereas RFP-2 xPH-G redistributes similarly to wild-type protein (D). Bar, 10 µm.