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Fig. 3. FRAP analysis of GFP-m53BP1. (A) Fluorescence recovery kinetics of GFP-m53BP1 in non-irradiated HeLa cells. Images before bleaching (–1.5 s), immediately after the photobleach event (0 s), and later in the time course. The photobleached region is indicated by an arrowhead. (B) Relative intensities are plotted versus time in seconds (n=10). (C) FRAP analysis of GFP-NLS serves as a control (n=12). (D) Photobleaching and recovery of GFP-m53BP1 focus (indicated by an arrowhead) induced by exposure to 10 Gy of X rays. (E) Relative intensities of the GFP-m53BP1 foci plotted versus time after photobleaching (n=8). Values represent means±s.d. Bars, 10 µm.