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Fig. 1. Filament and aggregate patterns after the production of wild-type or mutant GFAP in primary rat astrocytes (A-C) and Cos-7 cells (D-F). (A) Filamentous pattern, 1 day after transfection, wild-type GFAP. (B) Aggregate, 1 day after transfection, wild-type GFAP. (C) Aggregate, 1 day after transfection, mutant GFAP. (D) Filamentous (arrow) and aggregate (arrowhead) patterns formed by wild-type GFAP at 3 days after transfection. (E) Filamentous pattern formed by mutant GFAP at 1 day after transfection. (F) Aggregates formed by mutant GFAP at 3 days after transfection. In all images, transfected GFAP was visualized with SMI21 and a FITC-conjugated secondary antibody. Magnification 900x for all images.