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Fig. 2. Filamentous and irregular patterns after expression of wild-type (A,B) and mutant GFAP (C,D) in SW13Vim– cells. (A) Filamentous pattern, 1 day after transfection with wild-type. (B) Filamentous pattern with `diffuse' background 1 day after transfection with wild-type GFAP, visualized with ALD-10, an anti-panGFAP antibody, and a FITC-conjugated secondary antibody. (C) Irregular pattern, 1 day after transfection with mutant GFAP. (D) 3 days after transfection with mutant. GFAP was visualized with SMI21 and a FITC-conjugated secondary antibody. All images represent 1 µm confocal optical sections. Magnification 900x for all images.