Fig. 7. Sedimentation assays. Recombinant human wild-type GFAP (WT) and mutant GFAP (R239C) were purified and assembled individually or as a 1:1 (WT+R239C) mixture at 0.5 mg ml^–1. After assembly, the samples were centrifuged and the supernatant (S) and pellet (P) fractions analysed by SDS-PAGE.