Fig. 6. Localization of HsCYK4 and MKLP1 in HeLa cells. (A) HsCYK4 and MKLP1 localize to central spindle microtubules and the tips of astral microtubules in control cells (control) but not in siRNA-treated cells (RNAi). RhoA (red, in middle panels) accumulated at the equatorial cortex (arrows) in control cells. (B) Magnified images of the boxed areas of A. Arrows indicate tips of the astral microtubule (green) with accumulated HsCYK4 or MKLP1 (magenta). All images in A and B are X-Y images projected to one plane from serial optical sections. Bars, 15 µm (A); 5 µm (B).