Fig. 4. The genomic environment of Mash1. (A) A 2 Mb region surrounding the Mash1 gene on chromosome 10, with neighbouring genes annotated. (B) RT-PCR analysis of genes surrounding Mash1 in OS25 ES and NE cells. (C) Replication timing of genes surrounding Mash1 in ES and NE cells. Bar charts show the abundance of nascent DNA in each S-phase fraction (as a percentage of the total). (D) Positions of the 2 Mb region probes relative to nuclear periphery (probe positions shown annotated in A). Also shown are the positions of silent, late replicating genes, Neurod (NeuroD) and Sprr2 (SPRR2) and active, early replicating genes, Sox2 (Sox2) and Utf1 (UTF) as controls. Numbers beneath probes represent FISH signals scored in high-throughput analysis (blue, ES cells; pink, neural cells).