Fig. 7. Effect of GFP-ß2-chimaerin on CXCL12/PMA-induced adhesion of primary blood T lymphocytes. Human peripheral blood mononuclear cells were prepared, and T lymphocytes were isolated and nucleofected as described in the Materials and Methods. After 6 hours, cells were added to wells coated with sVCAM-1 alone (medium), or co-immobilized with CXCL12 and allowed to adhere (37°C, 2 minutes). Where indicated, cells were stimulated with PMA (100 ng/ml, 5 minutes) before addition to the wells. The inset shows endogenous ß2-chimaerin expression in cell lysates of Jurkat and hPBLs, determined by western blotting. Tubulin expression was measured as a control of total protein levels. Data represent the mean ± s.d. of two independent experiments performed in triplicate.