Fig. 7. A comparative experiment of gad mice and heterozygous littermates. The experiment compared gad mice (A,B) with a transfection study using FLAG-tagged WT UCH-L1, C90S UCH-L1, D30A UCH-L1 and GFP (mock) into gad-mouse-derived NPCs (C). The lengths of nestin-positive processes in immunostained cells were measured. NPCs from gad mice had longer nestin-positive processes compared with the control (A,B). (C) At 48 hours after transfection, bFGF was removed for 12 hours before the cultures were immunostained. The lengths of nestin-positive processes in immunostained cells were measured. Asterisks indicate differences from the value of GFP-transfected NPCs at ^**P<0.01 and ^****P<0.0001. Bar, 50 µm.