Fig. 2. Myo10 expression in brain is developmentally regulated. Western blot of whole cerebri (A) or cerebelli (B) from mice at postnatal days 1 to adult (P>45) were lysed in SDS sample buffer and immunoblotted with antibody no. 3568 against mouse Myo10. Both full-length- (240 kDa) and headless-Myo10 (164 kDa) are expressed in mouse cerebrum where they show clear developmental regulation, with a peak of expression between postnatal days 5-15. Cerebellum from mouse shows a different pattern of Myo10 expression, with prominent and increasing levels of full-length Myo10 expression throughout development. (C) Full-length and headless Myo10 (arrowheads) are detected by immunoblotting in undifferentiated CAD cells as well as in CAD cells differentiated into a neuronal phenotype.