Fig. 5. Full-length Myo10, but not headless Myo10, localizes to the tips of filopodia when transfected into CAD cells. (A) In undifferentiated CAD cells, full-length GFP-Myo10 (green) localizes to the tips of the short filopodia along the leading edge. (B) Headless GFP-Myo10 does not localize to filopodial tips and instead shows a more diffuse localization to the cell membrane and the central portion of undifferentiated CAD cells. (C) In CAD cells induced to differentiate into a neuronal phenotype, full-length GFP-Myo10 localizes to neurites as well as the tips of neuronal filopodia. (D) Headless GFP-Myo10 localizes primarily to the soma and neurites of differentiated CAD cells and does not localize to the tips of filopodia. (E) High-magnification images showing localization of full-length GFP-Myo10 to the tips of filopodia in CAD cells at early stages of differentiation. Notice that the filopodia frequently clump together at their tips. Cells were double-labeled with rhodamine-phalloidin to stain F-actin (red).