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Fig. 3. Laminin 5 does not modulate expression of ß-catenin and p120ctn in HT-29 cells. Western blot analysis of the total amount of ß-catenin (A) and p120ctn (B) during the time course of HT-29 cell culture onto laminin 5 matrix. Expression of actin was used as a loading control. Lower panel: the band densitometry was normalized with actin to allow quantification. Data indicate no significant variation in ß-catenin or p120ctn expression. Data represent mean ± s.e.m. of three independent experiments. P values determined by Student's t-test were not significant.