Fig. 8. PI 3-kinase signaling is necessary for adherens junction biogenesis. HT-29 cells were cultured for 24 hours on laminin 5 matrix and subsequently treated with 4 mM EGTA for 30 minutes in the absence or presence of 50 µM LY294002. The EGTA-containing medium was then replaced with Ca²⁺-containing medium for 60 minutes. Cells were fixed and stained with anti-E-cadherin (green) and TRITC-conjugated phalloidin (red) before confocal microscopy analysis on optical sections. Bars, 25 µm.