JCS -- Katoh et al. 119 (1): 56 Figure IG1

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Fig. 1. RhoG knockdown in HeLa cells. (A) Cell lysates from HEK 293T cells transiently transfected with the indicated plasmids were analyzed by immunoblotting with anti-Myc (left) and anti-Rac1 (right) antibodies. (B) Cell lysates from stable HeLa cell clones expressing pSilencer-RhoGa (RhoG RNAi) and pSilencer-RhoGb (Control RNAi), or cell lysate from parental HeLa cells were analyzed by immunoblotting with anti-Rac1 (top), anti-Dock180 (middle) and anti-Crk (bottom) antibodies. (C) The levels of endogenous RhoG and ELMO2 mRNAs in parental, control RNAi and RhoG-RNAi HeLa cells were shown by semiquantitative RT-PCR.