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Figure 2


Fig. 2. Transcriptional activity of 2-cell HSC clone embryos assessed by a BrUTP incorporation assay. (A) Incorporation of BrUTP was detected by a specific antibody in the nuclei (arrows) of cumulus clone embryos (left), HSC clone embryos (right) and parthenogenetic embryos (not shown) at 12 hours post-activation. (B) The fluorescent intensity in the nuclei, which depicts transcriptional activity, was not different between cumulus and HSC clone embryos (P>0.05), and both were at the control level (parthenogenetic embryos=100%).