Fig. 5. Precipitation of tetraspanins by digitonin. Biotin-labeled Hepa1-6 cells were lysed in 1% Brij 97 and the lysates were treated with digitonin (digit. +) or with vehicle alone as a control (-). After elimination of the precipitated material, the supernatants were used for immunoprecipitation using anti-CD81 mAbs MT81 and MT81w or anti-5 integrin (5-int.). The panels are from the same membranes and were given the same exposure.