Fig. 4. Bsk activation in hemocytes. Hemocytes were recovered from early third-instar larvae and stained for Bsk activation using anti-phosphorylated-JNK antibody (red). Cells were counter-stained with FITC-phalloidin to visualize their actin cytoskeleton (green). In the merged pictures, overlap of the two stainings is yellow. (A) He-Gal4 (B) UAS-Bsk^A-Y; He-Gal4 (C) UAS-Rac1; He-Gal4 (D) UAS-Rac1^Y40C; He-Gal4 (E) UAS-Rac1^F37A; He-Gal4. (F) Quantification of Bsk activity. Imagetrak was used to measure fluorescence intensity of at least 100 hemocytes from three different larvae. ^*, significant difference (Student's t-test, P<0.01) compared with the parental UAS and He-Gal4 strains.