Fig. 5. Bsk is necessary for Rac1-induced increases in circulating hemocytes. (A,C,E) GFP expression in sessile hemocytes larvae expressing (A) UAS-Rac1; He-Gal4 (C) UAS-BskIR/He-Gal4 (E) UAS-Rac1;UAS-BskIR/He-Gal4. (B,D,F) In respective larvae, hemocyte actin cytoskeleton was visualized with TRITC-phalloidin, the nucleus was stained with DAPI. Arrows indicate cells not expressing the transgene. The He-Gal4 driver flies also contain an UAS-GFPnls transgene. GFP was used to indicate transgene expression. GFP expression is not shown in these figures. (G) Hemocyte counts after overexpression of various UAS alleles. Hemocytes were counted from at least 15 individual larvae. (H) Determination of plasmatocyte diameter. The cell diameter of plasmatocytes from the various genotypes was measured on their x and y axes, the average of 25 hemocytes was plotted in µm. ^*, significant difference (Student's t-test, P<0.01) compared with the parental UAS and He-Gal4 strains.