JCS -- Sjöberg et al. 119 (10): 2025 Figure IG4

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Fig. 4. Tau localizes inside the nucleolus and partially colocalizes with pericentromeric ${alpha}$ -satellite DNA. Non-confocal conventional fluorescence microscopy was used to analyze total DNA distribution and colocalization of tau with ${alpha}$ -satellite DNA in human skin fibroblasts and HeLa cells. (A and F) Total DNA distribution in cells revealed with Hoechst 33258. (B,G) Subnuclear tau distribution detected with the Tau-1 monoclonal antibody. (C,H) Distribution of ${alpha}$ -satellite DNA repeats revealed by FISH using ${alpha}$ -satellite p82H plasmid as a probe. Merged images of tau with Hoechst 33258 are shown in panels D (human skin fibroblasts) and I (HeLa cells) and merged images of tau with pericentromeric ${alpha}$ -satellite DNA are shown in panels E (human skin fibroblasts) and J (HeLa cells). Bars, 10 µm.