Fig. 4. Localisation of KIFC5A during the phases of the cell cycle in NIH 3T3 fibroblasts. (A) NIH 3T3 cells were processed for immunofluorescence with an antibody against -tubulin (red) and an antibody against KIFC5A (green). DNA was visualised with Hoechst (blue). (See also Fig. S4, supplementary material.) Bars, 5 µm. (B) NIH 3T3 cells, transiently transfected with YFP-KIFC5A. Only overlays are shown, with -tubulin staining in red, YFP-KIFC5A in green and DNA in blue. In the left-most panel, visualising a field of cells in interphase, only one cell is transfected. YFP-KIFC5A localisation matches closely that of the endogenous KIFC5A (panels in A). Bars, 10 µm.