JCS -- Christodoulou et al. 119 (10): 2035 Figure IG5

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Fig. 5. Overexpression of KIFC5A inhibits bipolar spindle formation and causes stalling of mitotic progression in prometaphase. (A) Two typical examples of NIH 3T3 cells overexpressing YFP-KIFC5A (top panel) or myc-KIFC5A (bottom panel). Cells were labelled for ${alpha}$ -tubulin (red) or myc (green). DNA was visualised with Hoechst (blue), YFP-KIFC5A is green. Bars, 5 µm. (B) Another example of the `stalled phenotype' in prometaphase with the use of anti-pericentrin (top panel). In the overlay, YFP-KIFC5A is green, ${alpha}$ -tubulin staining is red and pericentrin is blue. A normal cell in prometaphase is shown for comparison (bottom panel). Bars, 5 µm.